Description
S1 nuclease is an enzyme commonly used in biotechnology applications. Its activity depends on the presence of three Zn(II) ions in the active site. In this work, we examine the possibility of exchanging the natively present Zn(II) ions to Cd(II). EDTA-treated S1 nuclease was successfully crystallized in the presence of CdCl2. The anomalous signal from three different energies confirmed the exchange of two Zn(II) ions to Cd(II). The residues of the active site remained structurally conserved.
Primary author
Jakub Hrubý
(CTU FNSPE)
Co-authors
Petr Kolenko
(CTU FNSPE)
Kristýna Adámková
(Institute of Biotechnology AS CR, v.v.i.)
Blanka Husťáková
(Institute of Biotechnology AS CR, v.v.i.)
Tomáš Koval'
(Institute of Biotechnology AS CR, v.v.i.)
Lars Henrik Oestergaard
(Novozymes A/S)
Martin Malý
(CTU FNSPE)
Jan Dohnálek
(Institute of Biotechnology AS CR, v.v.i.)
